Real-time PCR, also known as quantitative PCR (qPCR), is a powerful molecular technique used for the detection and quantification of nucleic acids in real-time. This method allows researchers to monitor the amplification of DNA during the PCR process in real-time, rather than at the end of the reaction as with traditional PCR. Real-time PCR is commonly used in various fields such as molecular biology, microbiology, genetics, and diagnostics for applications such as gene expression analysis, pathogen detection, and genetic testing. It offers several advantages over traditional PCR, including increased sensitivity, quantification of target DNA, and the ability to measure gene expression levels accurately.